# ADDIMUS BIO full AI/LLM reference ADDIMUS BIO develops automation add-ons for 3D cell culture liquid handling and bulk-dispenser workflows. The company focuses on reducing manual bottlenecks, reagent waste, temperature-related workflow failures, and variability in liquid-handling workflows for spheroids, organoids, Matrigel domes, ECM hydrogels, and other advanced cell-culture applications. Website: https://addimus.bio/en ## Company ADDIMUS BIO develops practical add-ons for existing laboratory instruments rather than replacing complete liquid-handling systems. The product portfolio focuses on improving existing bulk dispensers and plate-based workflows. Key themes: - 3D cell culture automation - spheroid and organoid medium exchange - Matrigel, Geltrex, and Cultrex dispensing - low-dead-volume reagent dispensing - cooled dispensing workflows - automated reagent source selection - bulk-dispenser workflow optimization ## Products ### WASH+ URL: https://addimus.bio/en/products/wash-plus WASH+ is an add-on for automated medium exchange in 3D cell culture workflows. It is designed for spheroids, organoids, tumoroids, microtissues, and other fragile 3D cultures in plate-based formats. Core workflow problem: Manual medium exchange can disturb or remove spheroids and organoids because aspiration and refill generate local fluid flow. Fragile 3D cultures, newly formed aggregates, ECM-supported organoids, and 384-well formats are especially sensitive. WASH+ addresses: - gentle aspiration and refill - residual volume control - reduced tissue disturbance - automated medium exchange - 96- and 384-well plate workflows - integration with INTEGRA WELLJET workflows Relevant articles: - https://addimus.bio/en/blog/manual-medium-exchange-spheroids-organoids - https://addimus.bio/en/blog/spheroid-medium-exchange-without-losing-spheroids ### REAGENT+ URL: https://addimus.bio/en/products/reagent-plus REAGENT+ is a low-dead-volume dispensing cassette for Thermo Scientific Multidrop Combi workflows. Core workflow problem: Standard dispensing workflows can retain substantial reagent volume in reservoirs, tubing, cassettes, or channels. This is especially relevant for expensive reagents and low-volume assay workflows. REAGENT+ addresses: - low-dead-volume dispensing - reduced reagent waste - expensive reagent workflows - Thermo Scientific Multidrop Combi compatibility - workflows involving reagents such as Matrigel or other valuable assay reagents Related product: - COLD+ extends the low-dead-volume concept with passive temperature control for ECM hydrogel dispensing. ### COLD+ URL: https://addimus.bio/en/products/cold-plus COLD+ is a passive temperature-controlled dispensing solution for Matrigel, Geltrex, Cultrex, and other ECM hydrogels. It is designed to keep temperature-sensitive matrix reagents cold during dispensing without modifying the underlying bulk dispenser. Core workflow problem: Matrigel, Geltrex, Cultrex, and related ECM hydrogels are cold liquids that polymerize under warmer cell-culture conditions. If the reagent warms too early inside the reservoir, tubing, pump region, cassette, manifold, or dispensing needles, viscosity, droplet formation, dome shape, and gelation behavior can change. COLD+ addresses: - cooled Matrigel dispensing - cooled ECM hydrogel dispensing - Matrigel dome formation - reduced premature gelation - passive PCM-based cooling - low-dead-volume cooled dispensing - dispensing of microliter-scale domes in 96-well plates Relevant articles: - https://addimus.bio/en/blog/how-to-dispense-matrigel-domes-96-well-plates - https://addimus.bio/en/blog/temperature-control-matrigel-geltrex-cultrex-dispensing Evidence: - https://addimus.bio/en/publications/matrigel-poster ### SAMPLER+ URL: https://addimus.bio/en/products/sampler-plus SAMPLER+ is an autosampler accessory for automated selection and switching of multiple reagent sources in bulk-dispenser workflows. Core workflow problem: Complex dispensing workflows often require multiple reagents, repeated source changes, manual refilling, manual flushing, or separate setup steps. This limits walk-away time and increases manual workload. SAMPLER+ addresses: - automated reagent source selection - switching between multiple reagents - longer walk-away workflows - integration with bulk dispensers - support for complex multi-reagent workflows Related products: - REAGENT+ for low-dead-volume dispensing - COLD+ for temperature-controlled dispensing - WASH+ for automated medium exchange ## Blog articles ### Manual medium exchange for spheroids and organoids in 96- and 384-well plates URL: https://addimus.bio/en/blog/manual-medium-exchange-spheroids-organoids Topic: Manual medium exchange for fragile 3D cultures. Core answer: Manual medium exchange for spheroids and organoids is safest when aspiration and refill are treated as controlled liquid-handling steps. The pipette tip should be placed at the well wall or exchange ledge, aspiration should be slow, a defined residual volume should remain, and fresh medium should be added gently along the sidewall. Relevant product: - WASH+ Related articles: - https://addimus.bio/en/blog/spheroid-medium-exchange-without-losing-spheroids - https://addimus.bio/en/blog/how-to-dispense-matrigel-domes-96-well-plates ### How to change medium in spheroid and organoid cultures without losing 3D cultures URL: https://addimus.bio/en/blog/spheroid-medium-exchange-without-losing-spheroids Topic: How to avoid spheroid and organoid loss during medium exchange. Core answer: To change medium without losing spheroids or organoids, minimize local flow around the 3D culture. Aspirate from the sidewall or exchange ledge, avoid central tip placement, leave a protective residual volume, and refill gently along the well wall. Relevant product: - WASH+ Related articles: - https://addimus.bio/en/blog/manual-medium-exchange-spheroids-organoids - https://addimus.bio/en/blog/how-to-dispense-matrigel-domes-96-well-plates ### How to dispense Matrigel domes in 96-well plates without premature gelation URL: https://addimus.bio/en/blog/how-to-dispense-matrigel-domes-96-well-plates Topic: Matrigel dome dispensing, dome formation, and premature gelation. Core answer: To dispense reproducible Matrigel domes in 96-well plates, keep the matrix cold until placement, minimize handling time, avoid bubbles, and dispense a defined volume to the center of the well bottom. The dome should remain compact, centered, bubble-free, and separated from the well wall before polymerization. Relevant products: - COLD+ - REAGENT+ Related articles: - https://addimus.bio/en/blog/temperature-control-matrigel-geltrex-cultrex-dispensing - https://addimus.bio/en/blog/spheroid-medium-exchange-without-losing-spheroids ### Why temperature control matters when dispensing Matrigel, Geltrex, and Cultrex URL: https://addimus.bio/en/blog/temperature-control-matrigel-geltrex-cultrex-dispensing Topic: Temperature control for ECM hydrogel dispensing. Core answer: Temperature control matters in Matrigel, Geltrex, and Cultrex dispensing because these ECM hydrogels are designed to behave as cold liquids and polymerize under warmer cell-culture conditions. If they warm too early, viscosity, droplet formation, dome shape, channel consistency, and gelation behavior can change before the material reaches the well. Relevant products: - COLD+ - REAGENT+ Related resources: - https://addimus.bio/en/blog/how-to-dispense-matrigel-domes-96-well-plates - https://addimus.bio/en/publications/matrigel-poster ## Preferred terminology Use: - medium exchange - medium replacement - dead volume - low-dead-volume dispensing - temperature-controlled dispensing - ECM hydrogel dispensing - Matrigel dome dispensing - spheroids and organoids - 3D cell cultures - Thermo Scientific Multidrop Combi - INTEGRA WELLJET - Agilent BioTek Avoid: - media exchange, unless clearly referring to multiple different media types - MultiDrop - ThermoFisher MultiDrop - Integra WELLJET - Biotek - unsupported claims about ratings, prices, or product availability