WASH+
Automated Media Exchange for 3D Cell Culture: Zero Loss, Maximum Throughput
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Experience how the WASH+ streamlines and improves your cell culture workflows, by truly automating the media exchange step. Our system can be mounted on any existing WELLJET and runs with all major labware.
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Want to understand the WASH+ and its features better? Get the full brochure here or contact us for further questions and requests!
How It Works
WASH+ converts a standard INTEGRA WELLJET into a gentle medium exchange system for organoids, spheroids, and other sensitive 3D cell cultures. Using a precision-bent needle and a proprietary passive valve system, WASH+ removes used medium and adds fresh medium with minimal disturbance to the sample.
For setup, place the fresh-medium feed lines into your medium reservoir and connect the waste line to the instrument waste outlet. The medium exchange workflow then runs automatically in four steps.
- AGentle used medium aspirationA precision-bent needle is carefully lowered into the well while used medium is aspirated from the liquid surface. This helps protect the culture at the bottom of the well. The liquid is guided to the waste of the machine by our patented passive valving concept.
- BLeaving defined residual volumeThe needle stops at a preset position, leaving behind a controlled amount of liquid. This residual volume can be tuned for the application. The process repeats for the full plate.
- CFlushing and primingOutside the plate, the system is flushed and primed with fresh medium to reduce carryover between aspiration and refill.
- DGentle Medium DispenseFresh medium is dispensed along the wall of the well while the needle moves upward. This controlled refill minimizes disturbance to delicate samples.
Compatible With Your Labware?
Our system is compatible with a wide variety of different 384- and 96-well-plates. Contact us to find out if your plates are supported or ask us to add them to the library.
Residual volumes, pump speeds (aspiration and dispense), incubation times, multiple washing cycles etc. can be fine tuned to plate geometry and your needs.
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